Identification of Lice Stored in Alcohol Using MALDI-TOF MS.
Identifieur interne : 000760 ( Main/Exploration ); précédent : 000759; suivant : 000761Identification of Lice Stored in Alcohol Using MALDI-TOF MS.
Auteurs : Hanene Benyahia [France] ; Basma Ouarti [France] ; Adama Zan Diarra [France] ; Mehdi Boucheikhchoukh [Algérie] ; Mohamed Nadir Meguini [Algérie] ; Makhlouf Behidji [Algérie] ; Ahmed Benakhla [Algérie] ; Philippe Parola [France] ; Lionel Almeras [France]Source :
- Journal of medical entomology [ 1938-2928 ] ; 2021.
Abstract
Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized. The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.
DOI: 10.1093/jme/tjaa266
PubMed: 33346344
Affiliations:
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Nadir" last="Meguini">Mohamed Nadir Meguini</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Veterinary and Agronomic Sciences, Mohamed Cherif Messaadia University, Souk-Ahras, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Institute of Veterinary and Agronomic Sciences, Mohamed Cherif Messaadia University, Souk-Ahras</wicri:regionArea><wicri:noRegion>Souk-Ahras</wicri:noRegion></affiliation></author><author><name sortKey="Behidji, Makhlouf" sort="Behidji, Makhlouf" uniqKey="Behidji M" first="Makhlouf" last="Behidji">Makhlouf Behidji</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Veterinary and Agronomic Sciences, Mohamed Cherif Messaadia University, Souk-Ahras, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Institute of Veterinary and Agronomic Sciences, Mohamed Cherif Messaadia University, Souk-Ahras</wicri:regionArea><wicri:noRegion>Souk-Ahras</wicri:noRegion></affiliation></author><author><name sortKey="Benakhla, Ahmed" sort="Benakhla, Ahmed" uniqKey="Benakhla A" first="Ahmed" last="Benakhla">Ahmed Benakhla</name><affiliation wicri:level="1"><nlm:affiliation>Department of Veterinary Sciences, Chadli Bendjedid University, El-Tarf, 36000, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Department of Veterinary Sciences, Chadli Bendjedid University, El-Tarf, 36000</wicri:regionArea><wicri:noRegion>36000</wicri:noRegion></affiliation></author><author><name sortKey="Parola, Philippe" sort="Parola, Philippe" uniqKey="Parola P" first="Philippe" last="Parola">Philippe Parola</name><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IHU-Méditerranée Infection, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Almeras, Lionel" sort="Almeras, Lionel" uniqKey="Almeras L" first="Lionel" last="Almeras">Lionel Almeras</name><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IHU-Méditerranée Infection, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Unité Parasitologie et Entomologie, Département Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, 19-21 Boulevard Jean Moulin, 13005 Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Unité Parasitologie et Entomologie, Département Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, 19-21 Boulevard Jean Moulin, 13005 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of medical entomology</title><idno type="eISSN">1938-2928</idno><imprint><date when="2021" type="published">2021</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized. The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.</div></front></TEI><affiliations><list><country><li>Algérie</li><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Benyahia, Hanene" sort="Benyahia, Hanene" uniqKey="Benyahia H" first="Hanene" last="Benyahia">Hanene Benyahia</name></region><name sortKey="Almeras, Lionel" sort="Almeras, Lionel" uniqKey="Almeras L" first="Lionel" last="Almeras">Lionel Almeras</name><name sortKey="Almeras, Lionel" sort="Almeras, Lionel" uniqKey="Almeras L" first="Lionel" last="Almeras">Lionel Almeras</name><name sortKey="Almeras, Lionel" sort="Almeras, Lionel" uniqKey="Almeras L" first="Lionel" last="Almeras">Lionel Almeras</name><name sortKey="Benyahia, Hanene" sort="Benyahia, Hanene" uniqKey="Benyahia H" first="Hanene" last="Benyahia">Hanene Benyahia</name><name sortKey="Diarra, Adama Zan" sort="Diarra, Adama Zan" uniqKey="Diarra A" first="Adama Zan" last="Diarra">Adama Zan Diarra</name><name sortKey="Diarra, Adama Zan" sort="Diarra, Adama Zan" uniqKey="Diarra A" first="Adama Zan" last="Diarra">Adama Zan Diarra</name><name sortKey="Ouarti, Basma" sort="Ouarti, Basma" uniqKey="Ouarti B" first="Basma" last="Ouarti">Basma Ouarti</name><name sortKey="Ouarti, Basma" sort="Ouarti, Basma" uniqKey="Ouarti B" first="Basma" last="Ouarti">Basma Ouarti</name><name sortKey="Parola, Philippe" sort="Parola, Philippe" uniqKey="Parola P" first="Philippe" last="Parola">Philippe Parola</name><name sortKey="Parola, Philippe" sort="Parola, Philippe" uniqKey="Parola P" first="Philippe" last="Parola">Philippe Parola</name></country><country name="Algérie"><noRegion><name sortKey="Boucheikhchoukh, Mehdi" sort="Boucheikhchoukh, Mehdi" uniqKey="Boucheikhchoukh M" first="Mehdi" last="Boucheikhchoukh">Mehdi Boucheikhchoukh</name></noRegion><name sortKey="Behidji, Makhlouf" sort="Behidji, Makhlouf" uniqKey="Behidji M" first="Makhlouf" last="Behidji">Makhlouf Behidji</name><name sortKey="Benakhla, Ahmed" sort="Benakhla, Ahmed" uniqKey="Benakhla A" first="Ahmed" last="Benakhla">Ahmed Benakhla</name><name sortKey="Meguini, Mohamed Nadir" sort="Meguini, Mohamed Nadir" uniqKey="Meguini M" first="Mohamed Nadir" last="Meguini">Mohamed Nadir Meguini</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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